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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Phosphorylated Erk P Erk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Phosphorylated Erk P Erk 4370, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Anti Phospho Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Phospho Erk P Erk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Mouse Anti Phospho Erk P Erk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Phosphorylated Erk P Erk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGHG1 regulates PCa cell growth via <t>MEK/ERK/c-Myc</t> pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, <t>phosphor-ERK,</t> and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and <t>p-ERK</t> (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.
Rabbit Antiphospho P44 42 Erk P Erk Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


IGHG1 regulates PCa cell growth via MEK/ERK/c-Myc pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, phosphor-ERK, and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and p-ERK (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.

Journal: BioMed Research International

Article Title: IGHG1 Regulates Prostate Cancer Growth via the MEK/ERK/c-Myc Pathway

doi: 10.1155/2019/7201562

Figure Lengend Snippet: IGHG1 regulates PCa cell growth via MEK/ERK/c-Myc pathway . PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, phosphor-ERK, and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and p-ERK (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. ∗ denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.

Article Snippet: Primary antibodies against IGHG1, MEK, phosphorylated-MEK (p-MEK), ERK, phosphorylated-ERK (p-ERK), c-Myc, p21, and Cyclin D1 were obtained from Santa Cruz Biotechnology, Santa Cruz, CA, USA.

Techniques: Immunohistochemistry, Expressing, Transfection, Western Blot, Control